But but I've gain insight from this training package and would make a practical attempt soon. With the scalpel, carefully cut a small square from the newly exposed mushroom tissue. Soak the grain for 24 hours in a bowl of water (use enough water to cover the grain completely. Hello! And if yes, what is a right method? Taking the mushroom by its base, carefully spit it in two. Most mushroom clean rooms have a HEPA filter installed to provide clean oxygenated air. These are my first mushrooms and they are doing awesome. The room also requires a work bench and storage shelves for incubating your jars of spawn. Other Blue Oyster products:Blue Oyster Grain Spawn - for indoor commercial growingBlue Oyster Plug Spawn - for smaller log projectsBlue Oyster 'Spray and Grow' Kit - introductory indoor grow kitBlue Oyster Log Kit - introductory outdoor grow kitOyster Cultures - for making your own spawn. (letting it dry on a cloth in the clean room, for example) The same question comes using your method, does it have to be fully dry for sterilization? :) All the best! Taking a drill, make an 8mm hole in each jar lid. If you prefer a firm or dryer texture, continue to cook Oysters until their liquid has reduced and cooked off and they begin to brown. Both methods above are to be performed within the clean room as in steps 6 and 7. Remove any jars showing signs of contamination (such as green mould) from the clean room. Pressure cook the jars at 15 psi for 60 minutes. I am now ready to mix with substrate (straw in this case). has reduced and cooked off and they begin to brown. Used for small to large enthusiast or commercial projects. Hi, the prepared jars i'm using are 1 year old. Place on a shaded shelf within the clean room to incubate. The grain should be fully colonised with mycelium in seven to ten days. Pleurotus ostreatus: A fast substrate colonizer with a strong blue/gray hue. If you prefer a firm or dryer texture, continue to cook Oysters until their. If you have any questions or concerns, please feel free to contact us. Oyster Mushroom Plug Spawn - (Pleurotus spp.) Equally, with seed and spawn propagation, I would suggest it should be drained then steamed to create less chances for contamination. These methods are all low tech (requiring only basic equipment), covering the pleurotus ostreatus (winter) and pleurotus pulmonarius (summer) varieties. Pleurotus ostreatus: A fast substrate colonizer with a strong blue/gray hue. Wash your hands with antibacterial hand-wash and in the clean room remove jars (while still warm), giving them a shake to allow the grain to flow freely. The more you order, the more you save (mix or match): the volume discount pricing below applies to large bags (4 lb.) For larger projects you may want to purchase an Angle Grinder Adapter and specialized 12mm drill bit! Used for small to large enthusiast or commercial projects. Depending on availability, you can buy medical grade cotton which is sterile. Thanks, Reply Clean your hands with antibacterial soap or wear sterile gloves. Hey Jen, yes you are right, however the key to a healthy uncontaminated culture is to reduce the exposure time during spore transfer. Take the sterilised grain jar, with cotton wool plugging its breathing hole and inject the inoculant past the cotton and into your grain. It is rich in protein andquite good of hair and eyes.After the suggestion of my friend, I got some idea about this from Agrinoon (Fujian). 100 % more water now - ( pleurotus spp. your water jar, before its sterilisation, helps cut. Believe it or not bought my Oysters @ Home Depot staples to attach ) help much! Only for lawful purposes lift the lid 's breathing oyster mushroom spawn and attach to sterilised! Or concerns, please feel free to contact us because during the last year water inside the jars. 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